AMH
ZECEN
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SUMMARY AND EXPLANATION OF THE TEST
The Anti-Miillerian Hormone is a substance secreted by the granulosa cells in the developing egg sacs (ovarian follicles). When a woman grows older, her number of ovarian follicles begins to decrease; therefore, the amount of AMH in the blood decreases. A woman who has very few remaining follicles will have low AMH levels and is close to menopause. A woman who has many small ovarian follicles — a condition known as polycystic ovarian syndrome (PCOS) — typically has high levels of AMH. The AMH test (Anti-Miillerian Hormone) is a blood test to help assess a woman's ovarian reserve.The test measures the levels of AMH in a woman's blood and is helpful in determining whether a woman has premature ovarian aging (diminished ovarian reserve) or premature ovarian failure.
PRINCIPLE OF THE TEST
Sandwich method: AMH present in the test sample is bound with monoclonal antibody immobilized on magnetic beads and enzyme-labeled monoclonal antibody. The magnetic beads are washed to remove unbound enzyme-labeled antibody and are then incubated with the substrate. The amount of enzyme-labeled monoclonal antibody that binds to the beads is directly proportional to AMH concentration in the test sample. A standard curve is constructed using known levels of calibrators, and unknown sample concentrations are calculated using this curve.
Storage and Stability
⚫ Sealed: Stored at 2-8° C until the expiry date.
⚫ Opened: Stable for 4 weeks.
⚫ Open kits should be used within 12 hours or refrigerated at 2-8 °C.
SUMMARY AND EXPLANATION OF THE TEST
The Anti-Miillerian Hormone is a substance secreted by the granulosa cells in the developing egg sacs (ovarian follicles). When a woman grows older, her number of ovarian follicles begins to decrease; therefore, the amount of AMH in the blood decreases. A woman who has very few remaining follicles will have low AMH levels and is close to menopause. A woman who has many small ovarian follicles — a condition known as polycystic ovarian syndrome (PCOS) — typically has high levels of AMH. The AMH test (Anti-Miillerian Hormone) is a blood test to help assess a woman's ovarian reserve.The test measures the levels of AMH in a woman's blood and is helpful in determining whether a woman has premature ovarian aging (diminished ovarian reserve) or premature ovarian failure.
PRINCIPLE OF THE TEST
Sandwich method: AMH present in the test sample is bound with monoclonal antibody immobilized on magnetic beads and enzyme-labeled monoclonal antibody. The magnetic beads are washed to remove unbound enzyme-labeled antibody and are then incubated with the substrate. The amount of enzyme-labeled monoclonal antibody that binds to the beads is directly proportional to AMH concentration in the test sample. A standard curve is constructed using known levels of calibrators, and unknown sample concentrations are calculated using this curve.
Storage and Stability
⚫ Sealed: Stored at 2-8° C until the expiry date.
⚫ Opened: Stable for 4 weeks.
⚫ Open kits should be used within 12 hours or refrigerated at 2-8 °C.
