LH
ZECEN
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INTENDED USE
The kit has been designed for the quantitative determination of Luteinizing Hormone (LH) in human serum. The method can be used for samples over the range of 0.15-200.00 mIU/mL. The test has to be performed Taizhou ZECEN Biotech Co., Ltd. model CIA 600, CIA 600Plus, CIA 1200, CIA 1200M, CIA 1800, CIA 2800 and POClia 8, POClia minus, POClia plus, POClia auto automatic chemiluminescence analyzer. Shenzhen IncreCare Biotech Co., Ltd model Shine i1900、Shine i2900 automatic chemiluminescence analyzer.
SUMMARY AND EXPLANATION OF THE TEST
Luteinizing hormone (LH) quantitative detection kit (CLIA) (hereinafter referred to as the kit) is used for the in vitro quantitative determination of luteinizing hormone (LH) levels in serum. It is of great significance for diagnosis, treatment monitoring and prognosis of male and female infertility, menstrual disorders and other ovary or testis-related diseases, Luteinizing hormone (LH) is a glycoprotein, containing α and βsubunits, with the molecular weight of approximately 28.5 kDa. α subunit of LH is similar to the α subunit of FSH, hCGand TSH, and β subunits is different from other glycoprotein hormonest with a unique biochemical properties.
PRINCIPLE OF THE TEST Sandwich method:
LH Antibody labeled by FITC and LH Antibody pair labeled by AP bind with the LH antigen in the sample, control or calibrator and form sandwich complex. Then add the magnetic beads binding with Anti-FITC, through the specific binding between the FITC and Anti-FITC, the complex will be bound by the magnetic beads. Thenthe entire complex will be captured by the external applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through software by comparing the luminous intensity with the cutoff value to determine whether the corresponding antibody exists.
INTENDED USE
The kit has been designed for the quantitative determination of Luteinizing Hormone (LH) in human serum. The method can be used for samples over the range of 0.15-200.00 mIU/mL. The test has to be performed Taizhou ZECEN Biotech Co., Ltd. model CIA 600, CIA 600Plus, CIA 1200, CIA 1200M, CIA 1800, CIA 2800 and POClia 8, POClia minus, POClia plus, POClia auto automatic chemiluminescence analyzer. Shenzhen IncreCare Biotech Co., Ltd model Shine i1900、Shine i2900 automatic chemiluminescence analyzer.
SUMMARY AND EXPLANATION OF THE TEST
Luteinizing hormone (LH) quantitative detection kit (CLIA) (hereinafter referred to as the kit) is used for the in vitro quantitative determination of luteinizing hormone (LH) levels in serum. It is of great significance for diagnosis, treatment monitoring and prognosis of male and female infertility, menstrual disorders and other ovary or testis-related diseases, Luteinizing hormone (LH) is a glycoprotein, containing α and βsubunits, with the molecular weight of approximately 28.5 kDa. α subunit of LH is similar to the α subunit of FSH, hCGand TSH, and β subunits is different from other glycoprotein hormonest with a unique biochemical properties.
PRINCIPLE OF THE TEST Sandwich method:
LH Antibody labeled by FITC and LH Antibody pair labeled by AP bind with the LH antigen in the sample, control or calibrator and form sandwich complex. Then add the magnetic beads binding with Anti-FITC, through the specific binding between the FITC and Anti-FITC, the complex will be bound by the magnetic beads. Thenthe entire complex will be captured by the external applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through software by comparing the luminous intensity with the cutoff value to determine whether the corresponding antibody exists.
