fE3
ZECEN
| Quantity: | |
|---|---|
PRINCIPLE OF THE TEST
Competing method: fE3 in samples, calibrators or positive control, competes with the fE3 labeled by FITC to bind the fE3 monoclonal antibody labeled by AP and form Ab-Ag complex. Then add the anti-FITC magnetic beads. Through the specific binding of anti-FITC antibody with FITC, the Ag-Ab complex will be occupied by the magnetic particles. The entire complex will be captured by the applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through s
oftware by comparing the luminous intensity with the cutoff value.
Storage and Stability
⚫ Sealed: Stored at 2-8℃ until the expiry date.
⚫ Opened: Stable for 4 weeks. To ensure best kit’s performance, it is recommended to place opened kits in the refrigerator if it’s not going to be used on board during next 12 hours.
PRINCIPLE OF THE TEST
Competing method: fE3 in samples, calibrators or positive control, competes with the fE3 labeled by FITC to bind the fE3 monoclonal antibody labeled by AP and form Ab-Ag complex. Then add the anti-FITC magnetic beads. Through the specific binding of anti-FITC antibody with FITC, the Ag-Ab complex will be occupied by the magnetic particles. The entire complex will be captured by the applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through software by comparing the luminous intensity with the cutoff value.
Storage and Stability
⚫ Sealed: Stored at 2-8℃ until the expiry date.
⚫ Opened: Stable for 4 weeks. To ensure best kit’s performance, it is recommended to place opened kits in the refrigerator if it’s not going to be used on board during next 12 hours.
