N-terminal Pro-Brain Natriuretic Peptide Diagnostic Test Cardiac Markers NT-proBNP Automated Immunoassay Analyzer

PRINCIPLE OF THE TEST:

Sandwich method:

NT-proBNP antibody labeled by FITC and NT-proBNP Antibody pair labeled by AP bind with the NT-proBNP antigen in the sample, control or calibrator and form sandwich complex. Then add the magnetic beads binding with Anti-FITC, through the specific binding between the FITC and Anti-FITC, the complex will be bound by the magnetic beads. Then the entire complex will be captured by the external applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through software by comparing the luminous intensity with the cutoff value to determine whether the corresponding antibody exists.