Glucose metabolism Diabetes indicator GAD65 detection kit on chemiluminescence immunoassay system
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Glucose metabolism Diabetes indicator GAD65 detection kit on chemiluminescence immunoassay system

Human glutamic acid decarboxylase 65 (hGAD65) is a key autoantigen in type 1 diabetes, an autoimmune disease resulting from the destruction of insulin-producing β cells in the pancreas
  • GAD65

  • zecen

  • DR1100

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Anti-glutamic acid decarboxylase antibody GAD-Ab

Glutamic acid decarboxylase is the key rate-limiting enzyme for the inhibitory neurotransmitter γ-aminobutyric acid. It is composed of two isomers, GAD65 and GAD67. Anti-GAD65 antibodies are the main target antigen of anti-GAD antibodies in type 1 diabetes. Anti-GAD antibodies are an important immune marker of insulin-dependent diabetes (type 1 diabetes). The positive rate of anti-GAD antibodies in prediabetes and type 1 diabetes is 70-90%, and the positive rate is higher in older children and late-onset type 1 diabetes.

Latent autoimmune diabetes in adults (LADA) is easily misdiagnosed as type 2 diabetes due to its insidious onset, slow progression, partial pancreatic β-cell function, and effective oral hypoglycemic drugs. Joint detection of anti-glutamic acid decarboxylase antibodies and anti-islet cell antibodies can help early identification of LADA.

In addition, anti-GAD antibodies are associated with stiff-person syndrome, with a positive rate of 60-100% in this disorder.


The kit has been designed for the quantitative determination of  Glutamic acid decarboxylase65 calibrator(GAD65) in human serum.  

The method can be used for samples over the range of 2-2000IU/mL

product compositionTwo points: LH
Six   points: ABCDEF
Testing method1. Treat the calibrator as the sample to be tested, and place it in the sample loading area of the instrument after mixing. Note that when mixing
Avoid air bubbles.
2. During the detection, the system detects the luminescence value (RLU value) of each calibration point, and calculates the luminescence value according to the nominal concentration and luminescence value.
The calibration curve is automatically fitted by the instrument. In order to ensure the reliability of the calibration curve, the determination of the calibrator should be
Make a double.
3. The generated calibration curve can be used for 28 days, but it should be re-calibrated after the following conditions:
 Change of batch number of supporting testing reagents;
Change of batch number of luminescent substrate
 The measured value of the quality control product is not within the scope of quality control or the test results are inconsistent with the clinical situation;
 System for major repairs or maintenance.
Interpretation of test results1. For reasons such as methodology or antibody specificity, the same sample was tested using reagents from different manufacturers.
Testing with different reagents may give different test results, therefore, the results obtained by testing with different reagents should not be directly
They are compared with each other in order to avoid false medical interpretations.
2. The quality control product can be used as a reference for the reliability of the current experimental results, and its measured value should be within the
within the range allowed by the quality control sheet. Test results should be based on reference value ranges and other clinical factors and results
Comprehensive judgment, when the test result is close to the upper limit or lower limit of the reference value range, it can be considered
samples for confirmation testing.
Storage and expiration date

Stored at 2-8°C, the product is valid for 12 months. Reagents should be used within 1 month after opening, and stored at 2-8°C away from direct sunlight.





Zecen Biotech CO., LTD. founded in 2011, is a leading Chinese diagnostics manufacturer specializing in in-vitro diagnostics devices and reagents.
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