Allergy Chemiluminescence reagent Detection Kit in Closed Analyzer
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Allergy Chemiluminescence reagent Detection Kit in Closed Analyzer

Product Introduction
Immunoglobulin E
(IgE) has a high affinity for basophils and mast cells, so it is also called reagin or cytophilic antibody. IgE is mainly produced by the plasma cells of the lamina propria of the respiratory tract and digestive tract mucosa, and its content is extremely low in the blood of normal people, accounting for about 0.002% of the total serum Ig. IgE is related to type I allergy. In patients with allergic constitution or hypersensitivity, the serum IgE is significantly higher than that of normal people, so the high level of IgE in serum often indicates the existence of genetic allergy or type I allergy.
  • Allergy


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Product Advantage


IgE is one of the body's 5 classes (isotypes) of immunoglobulins (antibodies).Like other immunoglobulins, IgE is produced by B cells and plasma cells. In contrast to other immunoglobulins, the concentration of IgE in the circulation is very low. Immunoglobulin E in cord blood usually measures less than 1 U/mL (1 U = 2.4 ng). Generally, adult IgE levels are achieved by 5 to 7 years of age. Between the ages of 10 and 14 years, IgE levels may be higher than those in adults. After age 70 years, IgE levels may decline slightly and be lower than the levels observed in adults younger than 40 years.

Circulating IgE concentrations are very low because mast cells have a very high affinity for IgE (1010 mol/L−1) via their -heavy-chain Fc receptors (FcER). The synthetic rate for IgE is also very low. Immunoglobulin E attaches to mast cells and to basophils and activated eosinophils. Immunoglobulin E on mast cells has a half-life (T½) of more than 10 days. In contrast to other immunoglobulins that bind to immunoglobulin Fc receptors only when antigen has been bound by an antibody, IgE will bind to FcER in the absence of antibody. Immunoglobulin E binding to mast cells “sensitizes” the mast cells to degranulate when multivalent antigens cross-link FcER-bound IgE. Intrinsically innocuous antigens that produce hypersensitivity responses are termed allergens.


Sandwich method:

IgEAntibody labeled by FITC and IgE Antibody pair labeled by AP bind with the IgE antigen in the sample, control or calibrator and form sandwich complex. Then add the magnetic beads binding with Anti-FITC, through the specific binding between the FITC and Anti-FITC, the complex will be bound by the magnetic beads. Then the entire complex will be captured by the external applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through software by comparing the luminous intensity with the cutoff value to determine whether the corresponding antibody exists.

Technical Parameters

Product Uses

Detection of the content of immune globulin in the body can understand the state of the body's humoral immune function and help diagnose various diseases such as immune hyperplasia, immune deficiency, infection and autoimmunity, which has important clinical significance.

1. Elevated serum IgE: common in hypersensitivity diseases such as allergic rhinitis, extrinsic asthma, hay fever, chronic urticaria, as well as parasitic infection, acute and chronic hepatitis, drug-induced interstitial pneumonia, bronchial Pulmonary aspergillosis, eczema, rheumatoid arthritis and IgE multiple myeloma, etc.

2. Decreased serum IgE: seen in primary agammaglobulinemia, after the application of tumor and chemotherapy drugs, ataxia-telangiectasia, etc.

Normal value reference range

Normal reference value of serum IgE content: <100IU/ml.

Product Operate Guide


The kit has been designed for the quantitative determination of Immunoglobulins E (IgE) in human serum. The method can be used for samples over the range of 0.25-3000.00 IU/mL.


1. Is joint testing necessary?

Yes. Choose according to the pregnant woman’s pregnancy cycle

2.Does Down syndrome software need to be purchased separately?


3. Is Tangsie software used in closed systems?

Yes, only applicable to analyzers produced by ZECEN

Zecen Biotech CO., LTD. founded in 2011, is a leading Chinese diagnostics manufacturer specializing in in-vitro diagnostics devices and reagents.
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